Another "what is this in my negatives?" submit

This is my first roll of Tri-X 400, developed with D76 using the Massive Dev table in this order:

  • developer
  • bathroom stop (tap water)
  • fixative
  • tap water (1 minute quick wash)
  • Photo Flo (2 minutes)
  • tap water (final wash, 11 minutes)

then turn off the scanner the next day (flatbed V500) to scan.

However, there are some strange effects, as well as the usual pixelated appearance that I have become accustomed to with 35mm flatbed scanning.

I have highlighted the points in the photos below:



I wonder what this is and how I can fix it. Using distilled water maybe?